Western Blot:Article Title: Targeting histone H2B acetylated enhanceosomes via p300/CBP degradation in prostate cancer.
Article Snippet: GNE-049, CCS1477, A485, JQAD1, enzalutamide, carfilzomib and thalidomide were purchased from Selleck Chemicals. dCBP-1 was purchased from MedChemExpress. .. For immunoblotting, the following antibodies were used: p300 (1:500; Invitrogen, MA1-16608); CBP (1:500; Invitrogen, PA5-27369); AR (1:1,000; Abcam, ab133273); H2BK5ac (1:1,000; Cell Signaling Technology, 12799S); H2BK20ac (1:1,000; Cell Signaling Technology, 34156S); H2BK12ac (1:1,000; Abcam, ab40883); H2BK16ac (1:1,000; Abcam, ab177427); H3K27ac (1:1,000; Cell Signaling Technology, 8173S); H3K18ac (1:1,000; Active Motif, 39755); H3K4me1 (1:1,000; Abcam, ab8895); H3K4me3 (1:1,000; Active Motif, 39060); H3K27me3 (1:1,000; Millipore, 07-449); H2B (1:1,000; Active Motif, 39210); H3 (1:1,000; Cell Signaling Technology, 3638S); H2BK120ac (1:1,000; Active Motif, 39119); Myc (1:1,000; Cell Signaling Technology, 9402S); KLK3/PSA (1:1,000; Dako, A0562); CITED2 (1:1,000; Abcam, ab108345); NKX3-1 (1:1,000; Cell Signaling Technology, 83700S); CCND1 (1:1,000; Abcam, ab16663); FOXA1 (1:1,000; Thermo Fisher Scientific, PA5-27157); Vinculin (1:1,000; Cell Signaling Technology, 18799S); GAPDH (1:1,000; Santa Cruz Biotechnology, sc-47724); BRD2 (1:1,000; Bethyl Laboratories, A700-008); BRD3 (1:1,000; Bethyl Laboratories, A302-368A); BRD4 (1:1,000; Bethyl Laboratories, A700-004CF); GSPT1 (1:1,000; Proteintech, 28130-1-ap); Aiolos (1:1,000; Cell Signaling Technology, 15103S); Ikaros (1:1,000; Cell Signaling Technology, 14859S); HDAC3 (1:1,000; Abcam, ab137704); goat anti-rabbit IgG (H+L)-horseradish peroxidase (HRP) conjugate (1:10,000; Biorad, 1706515); and ECL peroxidase (HRP) anti-mouse IgG (1:10,000; Cytiva, NA931-100UL). .. For ChIP-seq, the following antibodies were used: H3K27ac (1 μg per million cells; Diagenode, C15410196); p300 (2 μg per million cells; Abcam, ab14984); H2BK20ac (1 μg per million cells; Cell Signaling Technology, 34156S); H2BK5ac (1 μg per million cells; Cell Signaling Technology, 12799S); AR (2 μg per million cells; Millipore, 06-680); FOXA1 (2 μg per million cells; Thermo Fisher Scientific, PA527157); BRD4 (2 μg per million cells; Diagenode, C15410337); H3K18ac (1 μg per million cells; Active Motif, 39755); MED1 (2 μg per million cells; Active motif, 61065); ERG (2 μg per million cells; Cell Signaling Technology, 97249S); and Pol II (1 μg per million cells; Active motif, 39097).
Article Title: Targeting histone H2B acetylated enhanceosomes via p300/CBP degradation in prostate cancer
Article Snippet: GNE-049, CCS1477, A485, JQAD1, enzalutamide, carfilzomib and thalidomide were purchased from Selleck Chemicals. dCBP-1 was purchased from MedChemExpress. .. For immunoblotting, the following antibodies were used: p300 (1:500; Invitrogen, MA1-16608); CBP (1:500; Invitrogen, PA5-27369); AR (1:1,000; Abcam, ab133273); H2BK5ac (1:1,000; Cell Signaling Technology, 12799S); H2BK20ac (1:1,000; Cell Signaling Technology, 34156S); H2BK12ac (1:1,000; Abcam, ab40883); H2BK16ac (1:1,000; Abcam, ab177427); H3K27ac (1:1,000; Cell Signaling Technology, 8173S); H3K18ac (1:1,000; Active Motif, 39755); H3K4me1 (1:1,000; Abcam, ab8895); H3K4me3 (1:1,000; Active Motif, 39060); H3K27me3 (1:1,000; Millipore, 07-449); H2B (1:1,000; Active Motif, 39210); H3 (1:1,000; Cell Signaling Technology, 3638S); H2BK120ac (1:1,000; Active Motif, 39119); Myc (1:1,000; Cell Signaling Technology, 9402S); KLK3/PSA (1:1,000; Dako, A0562); CITED2 (1:1,000; Abcam, ab108345); NKX3-1 (1:1,000; Cell Signaling Technology, 83700S); CCND1 (1:1,000; Abcam, ab16663); FOXA1 (1:1,000; Thermo Fisher Scientific, PA5-27157); Vinculin (1:1,000; Cell Signaling Technology, 18799S); GAPDH (1:1,000; Santa Cruz Biotechnology, sc-47724); BRD2 (1:1,000; Bethyl Laboratories, A700-008); BRD3 (1:1,000; Bethyl Laboratories, A302-368A); BRD4 (1:1,000; Bethyl Laboratories, A700-004CF); GSPT1 (1:1,000; Proteintech, 28130-1-ap); Aiolos (1:1,000; Cell Signaling Technology, 15103S); Ikaros (1:1,000; Cell Signaling Technology, 14859S); HDAC3 (1:1,000; Abcam, ab137704); goat anti-rabbit IgG (H+L)-horseradish peroxidase (HRP) conjugate (1:10,000; Biorad, 1706515); and ECL peroxidase (HRP) anti-mouse IgG (1:10,000; Cytiva, NA931-100UL). .. For ChIP-seq, the following antibodies were used: H3K27ac (1 μg per million cells; Diagenode, C15410196); p300 (2 μg per million cells; Abcam, ab14984); H2BK20ac (1 μg per million cells; Cell Signaling Technology, 34156S); H2BK5ac (1 μg per million cells; Cell Signaling Technology, 12799S); AR (2 μg per million cells; Millipore, 06-680); FOXA1 (2 μg per million cells; Thermo Fisher Scientific, PA5-27157); BRD4 (2 μg per million cells; Diagenode, C15410337); H3K18ac (1 μg per million cells; Active Motif, 39755); MED1 (2 μg per million cells; Active motif, 61065); ERG (2 μg per million cells; Cell Signaling Technology, 97249S); and Pol II (1 μg per million cells; Active motif, 39097).
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